美国cospheric密度标记小球 DMB-kit

密度标记珠是已知质量密度的小色微球，用于校准密度梯度并确定梯度色谱柱的密度。密度梯度通常用于细胞，病毒和亚细胞颗粒的分离和纯化。通常使用覆盖一定密度范围的一组密度标记珠。

梯度是用在柱内不同高度漂浮的珠进行校准的。当一个测试样品被添加到色谱柱中时，它会下降到中性浮力点，密度由校准图确定。密度梯度柱可用于测量任何样品的密度。

化合物密度标记珠在2.5ml小瓶中以20％浓度提供在水溶液中。我们的密度珠粒尺寸在200-300um之间。与已停产的GE医疗保健套件不同，Cospheric的彩色微球已准备就绪，可直接使用。

运输：DMB产品不需要特殊运输。

储存：由于没有添加防腐剂，我们建议在批次编号为[YYMMDD-XXX]的6个月内使用非冷藏产品。该产品可以冷藏或冷冻以延长溶液保质期。密度珠是惰性的，不会降解。但是，该解决方案可能降解导致结块和悬浮不良，从而导致更宽的频带。如果溶液降解，可以将珠子冲洗干净，然后使用我们的Tween悬浮方案重新悬浮。

Density marker beads are small colored microspheres of known mass density that are used for calibrating density gradients and determining density in gradient columns. Density gradients are often used for separations and purification of cells, viruses and sub-cellular particles. Generally a set of several density marker beads covering a range of densities is used. 

The gradient is calibrated with beads that float at different heights within the column. When a test sample is added to the column it drops until the point of neutral buoyancy and the density is determined from the calibration chart. Density gradient columns can be used to measure the density of any sample.

Cospheric Density Marker Beads are provided at a 20% concentration in an aqueous solution in 2.5ml vials. Our Density Beads are in the 200-300um size range. Unlike GE Healthcare kits that have been discontinued, the Cospheric's colored microspheres are ready for use right out of the vial.

Shipping: DMB product does not require special shipping.

Storage: As no preservatives are added, we recommend unrefrigerated product to be used within 6 months of date coded lot# [YYMMDD-XXX]. The product can be refrigerated or frozen to extend the solutions shelf life. The density beads are inert, and do not degrade. However, the solution can degrade causing agglomeration and poor suspension, leading to wider bands. If the solution degrades, the beads can be flushed clean, and then re-suspended using our Tween Suspension Protocol.

Item #                 Description/Size 

DMB-kit                 Density Marker Beads Kit of 6 Vials (1.02, 1.04, 1.06, 1.08, 1.09, 1.13g/cc)

DMB-FGRN-1.02 2.5ml      Density Marker Beads for Density Gradients, Fluorescent Green Microspheres, 1.02g/cc, 20% concentration in Aqueous Solution

DMB-FORG-1.04 2.5ml      Density Marker Beads for Density Gradients, Fluorescent Orange Microspheres, 1.04g/cc, 20% concentration in Aqueous Solution

DMB-FVIO-1.06 2.5ml      Density Marker Beads for Density Gradients, Fluorescent Violet Microspheres, 1.06g/cc, 20% concentration in Aqueous Solution

DMB-RBLU-1.08 2.5ml      Density Marker Beads for Density Gradients, Dark Blue Microspheres, 1.08g/cc, 20% concentration in Aqueous Solution

DMB-FRED-1.09 2.5ml      Density Marker Beads for Density Gradients, Fluorescent Red Microspheres, 1.09g/cc, 20% concentration in Aqueous Solution

DMB-FBLU-1.13 2.5ml Density Marker Beads for Density Gradients, Fluorescent Blue Microspheres, 1.13g/cc, 20% concentration in Aqueous Solution